Phnshp45
Webb27 feb. 2024 · Fitness Effects of mcr-1 Gene and mcr-1 Plasmids in Vitro. The impact of plasmids pHNSHP45, pHNSHP45-2, pHNSHP23, pHNSHP17 and pHNSHP24 on host … Webb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆
Phnshp45
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WebbPlasmid Transformation Assays. The competent cells including E. coli DH5α containing pUC19-mcr-1 and E. coli C600+pHNSHP45 were prepared and used for transformation … Webb17 jan. 2024 · IncHI2 plasmids, such as pHNSHP45–2, have been frequently associated with global spread of mcr-1 genes (3,13,14). Figure 1 Sequence comparison of scaffolds …
Webb24 maj 2016 · In particular, the pHNSHP45 3 is the only one mcr-1-carring plasmid with known genome sequence prior to our study. Unlike the other mcr-1 -positive plasmid … Webb5 juli 2024 · pHNSHP45 was used as a reference to compare with the pMCRs described here and with other IncI2 plasmids. The outer circle with red arrows indicates annotation of the reference sequence. Gaps in the inner circles are missing regions when compared with the reference. Plasmids characteristics are included in Table 3.
WebbThe PCR based mapping of mcr-1 genetic context in our isolates revealed that almost all amplified genes were similar to pHNSHP45, except the truncated tnpA gene, missing IsApl1. Globally, plasmid analysis of various Incompatibility types has shown that IsApl1 is usually absent in IncX4 type plasmid while in Incl2 type plasmid, it is either absent or … WebbCRISPR/Cas9 system can efficiently eliminate plasmid from E. coli, the control vector pCas9, and the engineered vector pCas9-m1 were transformed into competent E. coli …
Webb6 feb. 2024 · Except for a 3,684 bp region that was also present in a pHNSHP45-2-like plasmid and included the ISApl1 and mcr-1, the entire nucleotide sequence showed >99.9% identity to the corresponding region ...
Webb10 jan. 2024 · The plasmid pHNSHP45 was extracted from E. coli K12 C600/pHNSHP45 using the EZNA ® plasmid mini kit I (Omega) and then electroporated into the E. coli K12 … hemoglobin and hemocyanin quizletWebbSequence alignment of seven IncI2-type mcr-1-bearing plasmids. pHNSHP45 with GenBank no. KP347127 was used as a reference to compare with other mcr-1-bearing plasmids … lane kiffin usc coachWebbEuropean Centre for Disease Prevention and Control (ECDC), European Food Safety Authority (EFSA) and European Medicines Agency (EMA). ECDC/EFSA/EMA first joint report on the integrated analysis of the consumption of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from humans and food-producing animals. hemoglobin and hemocyanin are bothWebb15 feb. 2024 · pHNSHP45 KP347127 I2 E. coli Pig China [1] pEC019 KY471145 I2 E. coli Chicken Korea [2] pEC111 KY471146 IncX4 E. coli Pig Korea [2] pEC006 KY471144 I2 E. … lane kitchen essentials buffetWebb6 mars 2024 · Generally, this plasmid has 31 an average GC% value of 46.29%, and is predicted to contain 548 putative 32 open reading frames (ORFs). Importantly, … lane landrith city of boulderWebb6 juli 2024 · Relative to the paradigm mcr-1-harboring IncI2-type plasmid pHNSHP45, pGD17-2 exhibited 51% coverage and 99% identity and possessed two insertion sequences (IS629 and IS1294) in the nikB gene, as well as an additional IS629-mediated insertion sequence (~56 kb) in the pilO gene (Fig. 1 and Additional file 2: Figure S2). hemoglobin and hematocrit values pertain toWebb15 mars 2024 · The pHNSHP45 plasmid-encoded PcnR protein favours the fitness of bacteria carrying mcr-1-bearing IncI2 plasmids. (A) PcnR enhances the fitness of E. coli … hemoglobin and hemocyanin