Slp-mcherry
WebbsLP-mCherry protein across the membrane. The number of red-labeled cells in the co- culturesystemwasabout13.9%asquantifiedbyflowcytometry(Figure3C).Asindicated WebbProtéine soluble sLP-mCherry A B Figure 1. Méthodes de marquage in vivo des cellules de la niche métastatique situées au voisi-nage des cellules tumorales. A. Les cellules cancéreuses donneuses (orange) sécrètent des vési-cules extracellulaires (EV) contenant l’ARNm codant la recombinase Cre. L’incorporation de ces
Slp-mcherry
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WebbLIPSTIC utilizes a bacterial sortase-A that can tag molecularly tag predefined cell-cell interactions of interest and can be analyzed via flow cytometry ex vivo (Pasqual et al., 2024).... WebbmCherry (Plasmid #176016) Print Enlarge View all sequences Purpose Mammalian expression of mCherry. Parton lab clone DFQ Depositing Lab Rob Parton Publication Lo …
WebbTo investigate their role in IPF, basal cells were isolated from IPF explant and healthy donor lung tissues. Single-cell RNA sequencing was used to assess differentially expressed … Webb15 mars 2024 · (A) The sLP-mCherry system is used for studying local interactive cellular composition for a given cell type. The signal-emitting cells expressing a secreted fluorescent protein can be further taken up by nearby signal-receiving cells, facilitating identification of interactive cell compositions.
Webb6 jan. 2024 · sLP-mCh是脂溶性标签连接mCherry的融合荧光蛋白（Ombrato et al., Nature 2024）。 sLP-mCh在供体细胞中表达后，会从供体细胞中释放出去，并进入邻近细胞， … WebbWe engineered MDA231-BrM and HCC1954-BrM cells to constitutively express, in addition to the cell-autonomous GFP, a cell membrane-permeable mCherry protein (sLP …
Webb28 aug. 2024 · Upon uptake, sLP-mCherry fluorescence has an intracellular half-life of 43h ( Extended Data Figure 1f) and is localized in CD63 + multi-lamellar bodies (lysosomal-like structures), where, due to its high photostability 5, it retains high fluorescent intensity ( Extended Data Figure 1g, h ).
Webb7 sep. 2024 · RK13 cells expressing SLP-mCherry-K3L (RK13-K3L) were generated by transfecting 3×10 5 RK13-E3K3 cells (Brennan et al., 2014) with 2.5 µg piggybac vector (see supplemental sequences) and 0.5 µg transposase vector (gift from Ed Grow, University of Utah) with 9 µL Fugene-HD. raymour and flanigan kitchen pantryWebbWe engineered MDA231-BrM and HCC1954-BrM cells to constitutively express, in addition to the cell-autonomous GFP, a cell membrane-permeable mCherry protein (sLP … raymour and flanigan kitchen hutchWebb21 maj 2024 · MCherry + niche-labelling lentivirus infected organoids were injected into the anterior lobe of prostate of 6- or 8-week-old C57BL/6 mice with around 2,400 organoids … simplify simplify nytWebb1 mars 2024 · Basal cell and niche interaction was demonstrated with the sLP-mCherry niche labeling system. Luminex assays were used to assess cytokines secreted by basal cells. The role of basal cells in fibroblast activation was studies. 3D organoid culture assays were used to interrogate basal cell effects on AEC2 renewal capacity. simplify sign mathWebb4 mars 2024 · Les cellules cancéreuses métastatiques donneuses (bleues) expriment des molécules liposolubles fluorescentes (sLP-mCherry, en rouge), qui sont incorporées, par diffusion passive, dans les cellules adjacentes aux cellules cancéreuses. Dans le texte Figure 2. Changements cellulaires dans la niche métastatique. A. simplify sign inWebbUpon uptake into a cell, sLP mCherry fluorescence has an intracellular half-life of 43 hours and is localised in CD63+ multi-lamellar bodies (lysosomal-like structures) where, owing … simplify sh trm treas etfWebb17 nov. 2024 · (A) The sLP-mCherry system is used for studying local interactive cellular composition for a given cell type. The signal-emitting cells expressing a secreted … simplify show work